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. 2020 Jun 19;11:3123. doi: 10.1038/s41467-020-16972-5

Fig. 2. TRAK1 increases KIF5B processivity.

Fig. 2

a, b Survival probability (Kaplan–Meier estimation) of the run length and interaction time, respectively, of KIF5B-GFP in presence of mCherry-TRAK1 (magenta, n = 189 molecules, N = 3 experiments) and mCherry-TRAK1∆ (cyan, n = 282 molecules, N = 3 experiments) (p = 0.0003 and p < 10−4, respectively). c Histograms of the average velocities of KIF5B-GFP in presence of mCherry-TRAK1 (magenta, n = 189 molecules, N = 3 experiments) and mCherry-TRAK1∆ (cyan, n = 282 molecules, N = 3 experiments), respectively. d, e Survival probability of the run length and interaction time, respectively, of KIF5B∆-GFP (green, n = 499 and 534 molecules, respectively, N = 2 experiments), KIF5B∆-GFP in complex with mCherry-TRAK1 (magenta, n = 222 and 221 molecules, respectively, N = 4 experiments) and KIF5B∆-GFP in complex with mCherry-TRAK1∆ (cyan, n = 82, N = 2 experiments). In presence of mCherry-TRAK1, the median run length and interaction time of KIF5B∆-GFP increased (both p-values < 10−4). The increase was significantly less pronounced in presence of mCherry-TRAK1∆ (p = 0.0008 and p = 0.0418, respectively). f Histograms of the average velocities of KIF5B∆-GFP (green, n = 534 molecules, N = 2 experiments), KIF5B∆-GFP in presence of mCherry-TRAK1 (magenta, n = 487 molecules, N = 4 experiments) and KIF5B∆-GFP in presence of mCherry-TRAK1∆ (cyan, n = 82 molecules, N = 2 experiments). The velocity of KIF5B∆-GFP decreased only in presence of mCherry-TRAK1 but not in presence of mCherry-TRAK1∆. See also Supplementary Fig. 2. All two-tailed p-values were obtained by a log-rank test. Source data are provided as a Source data file.