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. 2020 Jun 19;11(6):476. doi: 10.1038/s41419-020-2555-4

Fig. 3. YBX1 targets p110β to induce autophagy with p110β/Vps34/beclin1 activation.

Fig. 3

H1299 transfected by YBX1-siRNA and A549 transfected by YBX1-overexpression plasmids, (a) The level of AKT, p-AKT(Ser473), mTOR, p-mTOR(Ser2448), p70s6k, p-p70s6k (Thr389), (b) p110β and beclin1 proteins in H1299 and A549 cells were analyzed by western blot, (c) The expression of the p110β mRNA was analyzed by RT-PCR. d YBX1 was predicted to bind the promoter of p110β and the data from the transcription binding site analysis(Jaspar), and the predicted binding sites of YBX1 on the promoter of p110β (score >7) were presented. e YBX1 proteins in the nuclear protein-p110β probe-streptavidin bead complexes were detected by western blot using an anti-YBX1 antibody in NSCLC cells. f A549 cells were transfected with YBX1-overexpression or vector plasmids, then added actinomycin D(5 μg/mL) at 0, 1, 2, 3, 4 h. The expression of the p110β mRNA was analyzed by RT-PCR. Data represent the mean ± SD of three experiments, *P < 0.05, **P < 0.01 vs. control. g H1299 cells were transfected with YBX1 sh-NC or sh-RNA overnight prior to transfection with the p110β control vector or overexpression plasmids. A549 cells were treated with the control vector or YBX1-overexpression plasmids overnight and then treated with p110β si-NC or siRNA for 48 h. The expression of YBX1, p110β, and LC3I/II was detected by Western blot.