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Generation of Il2rg-mutated rabbits with the CRISPR/Cas9 system.
| Cas9 RNA (ng/μL) | guide RNA (ng/μL) | No. Oocyte injected | No. (%) Transferred embryo | No. (%) Pups born | No. (%) Mutants |
|---|---|---|---|---|---|
| 100 | 50 | 293 | 241 (82.3) | 22 (9.1) | 8 (36.3) |
| 200 | 100 | 259 | 194 (74.9) | 8 (4.1) | 6 (75) |
| total | 552 | 435 (78.8) | 30 (6.8) | 14 (46.7) | |
