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. 2020 Jun 19;10:9989. doi: 10.1038/s41598-020-66866-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SPME-GC-MS analysis of terpene products from assays with recombinant TPS enzymes. Partially purified proteins were incubated in the presence of GDP or (E,E)-FDP. 1: β-myrcene*, 2: limonene*, 3: α-terpinolene*, 4: (Z)-β-ocimene*, 5: (E)-β-ocimene*, 6: γ-terpinene*, 7: linalool*, 8: α-phellandrene*, 9: α-pinene*, 10: sabinene*, 11: α-terpineol*, 12: germacrene D*, 13: β-elemene, 14: δ-elemene, 15: nerolidol*, 16: (E)-α-bergamotene*, 17: (E)-β-farnesene*, 18: (Z)-α-bisabolene*, 19: β-bisabolene*, 20: sesquiphellandrene*, 21: (E)-α-bisabolene*. *indicates compounds that were identified with authentic standards or by comparison with compounds of Opopanax oil. Other compounds were identified by library comparison only (≥95% confidence level). Mass spectra of these compounds are shown in Supplementary Figure S12. Lower case letters indicate additional terpene compounds with lower identification confidence levels. Mass spectra of these compounds are depicted in Supplementary Figure S13. Note that retention times of products obtained from TPS-a enzymes with GDP are shifted by 1 min due to difference in column condition. B; SPME fiber-related background occurring in some assays.