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. 2020 Jun 19;10:9963. doi: 10.1038/s41598-020-66900-2

Figure 4.

Figure 4

Capsaicin negatively affects Drosophila health and lifespan. (a) Capsaicin impaired the survival of progenies. Fly eggs were transferred to fly food with different doses of capsaicin, and the percentage of progenies that pupated and eclosed was assessed, respectively. 3 replicates. (b) The timing of pupa formation and emergence of adult progenies. The numbers of pupa formation and adult emergence were recorded each day post egg laying. 3 replicates. (c,d) The lifespan of flies was shortened by oral capsaicin. 80 mM capsaicin was used. n = 20, 4 replicates. (e,f) The lifespans of flies were decreased by contact capsaicin. n = 5, 4 replicates. (g,h) Capsaicin decreased the locomotion of flies. Flies were treated with capsaicin, and the velocity of climbing and running was assayed. 80 mM was used. n = 5, 4 replicates. The independent samples t-test was used to assess the mean deviation of each column. Mean ± S.E.M.; symbols: NS p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001.