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. 2020 Jun 19;11(6):475. doi: 10.1038/s41419-020-2550-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The genomic location of the RP11-142A22.4. b The full-length RP11-142A22.4 was achieved using 5’ and 3’ rapid amplification of cDNA end reactions. The bands (arrows) for RP11-142A22.4 are indicated. c RP11-142A22.4 sequences were cloned between Rluc and Luc reporter genes with independent start and stop codons. d The relative luciferase activity of Luc/Rluc in the above vectors was tested. e RNA FISH for RP11-142A22.4. Nuclei were stained with DAPI. Scale bar = 20 µm. f Relative RP11-142A22.4 expression levels in nuclear and cytosolic fractions of preadipocytes. Scale bar = 20 µm. Data are presented as means ± SD; significant difference was identified with Student’s t test. *P < 0.05; **P < 0.01; ns, not significant.