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. 2020 Jun 19;11(6):475. doi: 10.1038/s41419-020-2550-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a RP11-142A22.4 expression in adipose tissues from 60 obese patients and 40 lean patients. b Pearson correlation between RP11-142A22.4 expression and BMI in the adipose tissues of 60 obese patients. c ROC curve for RP11-142A22.4 that indicates a diagnostic value for this lncRNA in obese patients. d Valuation of RP11-142A22.4 expression after siRNA treatment using qRT-PCR. e, f C/EBP-α and PPAR-γ expression was quantified using qRT-PCR after RP11-142A22.4 knockdown during preadipocytes differentiation. g Oil red O staining of differentiated preadipocytes after transfection with RP11-142A22.4 specific siRNA versus scramble controls. Scale bar = 100 µm. h C/EBP-α and PPAR-γ expression was quantified using western blot assay after RP11-142A22.4 knockdown during preadipocytes differentiation. Data are presented as means ± SD; significant difference was identified with Student’s t test. *P < 0.05; **P < 0.01; ns, not significant.