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. 2020 Jun 19;11(6):475. doi: 10.1038/s41419-020-2550-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a RIP experiments were performed using an antibody against AGO2 on extracts from preadipocytes. b LncRIP was performed using a RP11-142A22.4-specific probe and control probe in preadipocytes from obesity patients. The enrichment of RP11-142A22.4 and microRNAs was detected by RT-qPCR and normalized to the control probe. c Co-localization between RP11-142A22.4 and miR-587 was observed by RNA in situ hybridization in preadipocytes. Nuclei were stained with DAPI. Scale bar = 20 µm. d Schematic showing the predicted miR-587 sites in RP11-142A22.4. A Luciferase assay where preadipocytes were co-transfected with a scrambled control, miR-587 mimic, and a luciferase reporter plasmid containing wild-type RP11-142A22.4 (RP11-142A22.4-WT) (e) or mutant RP11-142A22.4 (RP11-142A22.4-mut) (f). g Expression of miR-587 was analyzed using RT-qPCR following RP11-142A22.4 knockdown. h RT-qPCR showed the level of RP11-142A22.4 in the streptavidin-captured fractions from the preadipocytes lysates after transfection with biotinylated miR-587 or control RNA. i Pearson correlation between RP11-142A22.4 expression and miR-587 expression in the adipose tissues of 60 obese patients using qRT-PCR. Data are presented as means ± SD; significant difference was identified with Student’s t test. *P < 0.05; **P < 0.01; ns, not significant.