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. 2020 Jun 19;11(6):475. doi: 10.1038/s41419-020-2550-9

Fig. 5. RP11-142A22.4 promotes adipogenesis through the miR-587/Wnt5β pathway.

Fig. 5

qRT-PCR quantification of Wnt5β expression after miR-587 knockdown (a) or RP11-142A22.4 overexpression (b). Wnt5β expression after transfection with RP11-142A22.4-specific siRNAs (c) or with miR-587 mimic was quantified with qRT-PCR (d). Luciferase assay where preadipocytes were co-transfected with a scrambled control, miR-587 mimic, and a luciferase reporter plasmid containing either wild-type Wnt5β (Wnt5β-wt) (e) or a Wnt5β construct with mutated miR-587 binding sites (Wnt5β-mut) (f). g, h Reversion assays using vectors overexpressing or knocking down RP11-142A22.4, as well as miR-587 mimics or inhibitors. Data are presented as means ± SD; significant difference was identified with Student’s t test. *P < 0.05; **P < 0.01; ns, not significant.