Fig. 4. Temporal generation of striatal projection neurons is altered in Nolz1^−/− mutant embryos.

a Expression of striatopallidal-specific markers Drd2 and SIX3 at the expense of striatonigral marker EBF1 in E11.5 Nolz1^−/− mutant embryos. Coronal sections of the striatum showing Drd2 expression by in situ hybridization and EBF1⁺DAPI⁺ and SIX3⁺bGAL⁺ double labelled cells by immunofluorescence. Note the selective upregulation of SIX3 in cells lacking NOLZ1 expression indicated by bGAL in Nolz1^bgal/bgal mutants. b Characterization of the expression profile of striatopallidal-specific markers in E12.5 Wt and Nolz1^−/− mutant embryos by in situ hybridization. c Graphs showing the percentage of SIX3⁺ cells versus DAPI (left graph) and bGAL labelled (right graph) cells in E15.5 Nolz1^bgal/+ and Nolz1^bgal/bgal mutant striatum. Mean values ± standard deviation; n = 3 biologically independent samples; SIX3⁺/DAPI⁺: Two-sided, unpaired T-test ***p = 0.00273, SIX3⁺ /bGAL⁺: Two-sided, unpaired T-test ***p = 6.79688xE⁻⁰⁵. d Percentage of BrdU labelled cells in E15.5 Wt and Nolz1^−/− mutant striatum after being injected with BrdU at different time points (E10.5, E11.5 or E12.5). Mean values ± standard deviation; n = 3 biologically independent experiments; Two-sided, unpaired T-test *p = 0.0111. e Graph showing percentage of FOXP1 expressing BrdU labelled cells in striatum of E15.5 Wt and Nolz1^−/− mutant embryos injected with BrdU at E10.5, E11.5 or E12.5. Mean values ± standard deviation; n = 3 biologically independent experiments; Two-sided, unpaired T-test *p = 0.02795. f Immunofluorescence analysis of FOXP1 and BrdU expression in coronal sections of E15.5 Wt and Nolz1^−/− mutant striatum injected with BrdU at different time points (E10.5, E11.5 or E12.5). Data are representative of three independent experiments (a, b, f). Scale bar in a–f, upper panel: 200 μm; Scale bar in f lower and right panels: 100 μm. Source data are provided as a source data file.