FIGURE 2.

Effects of UDCA, LCA, and 6‐MUDCA on disease activity in DSS‐treated mice. Starting 24 hr prior to administration of DSS (2.5% in the drinking water), and daily thereafter, separate groups of male C57BL6 mice received either endotoxin‐free PBS or Na⁺‐UDCA (30 mg/kg), Na⁺‐LCA (30 mg/kg), or Na⁺‐6α‐MUDCA (30 mg/kg) dissolved in PBS by IP injection. (a) Disease activity index (DAI) was assessed daily (n = 12). For clarity, the inset depicts DAI at the end of the treatment period on Day 7. (b) Body weight was assessed daily to monitor disease progression. (c) Mice were sacrificed on day 7 and their colons were removed and measured (n = 5–6). ***p < .001 compared to controls (no DSS treatment); ^## p < .001 compared to DSS‐treated mice