Table 1.

Decellularization protocols for rat lungs and larger lungs from swine, monkeys, and humans

Decellularization protocol for rat lungs
	Solution	Concentration	Perfusion flow	Duration
1	SDS	0.1% (wt/vol)	Gravity pressure of 80 mmHg	120 min
2	Distilled water		Gravity pressure of 80 mmHg	15 min
3	Triton X-100	1% (vol/vol)	Gravity pressure of 80 mmHg	10 min
4	PBS		Roller pump flow of 4 ml/min	2–3 days

Decellularization protocol for lungs from swine, monkeys, and humans
	Solution	Concentration	Perfusion flow	Duration
1	SDS	0.5% (wt/vol)	Constant pressure of 10–15 mmHg with roller pump	30 h
2	Distilled water		Constant pressure of 10–15 mmHg with roller pump	12 h
3	Triton X-100	1% (vol/vol)	Constant pressure of 10–15 mmHg with roller pump	12 h
4	PBS		Constant pressure of 0–15 mmHg with roller pump	2–3 days

SDS sodium dodecyl sulfate, PBS phosphate buffered saline