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. 2020 Apr 9;14(7):1731–1742. doi: 10.1038/s41396-020-0641-3

Fig. 3. Similar adsorption rates of ϕH20-like phages to ΔH20, ΔvanT ΔH20, and ΔvanO ΔH20 mutant cells.

Fig. 3

Exponentially growing cultures of the indicated strains at OD600 = 0.6–0.9 were mixed with a lysate of ϕH20-like phage p41. At the indicated time points, unadsorbed phage enumerated by plaque assay. Shown is the average of three independent experiments. Error bars indicate the standard error of the mean (N = 3). Adsorption rate constants (4.0 × 10−11 ± 0.8 × 10−11 PFU × ml per minute per bacterium for the ΔH20 mutant that is wildtype with regards to the QS system, 4.2 × 10−11 ± 1.7 × 10−11 for the ΔvanT ΔH20 mutant, and 2.9 × 10−11 ± 0.5 × 10−11 for the ΔvanO ΔH20 mutant) are not significantly different (linear regression analysis with Tukey adjustment, P = 0.16, α = 0.05).