Fig. 4. Extracellular proteolytic activity reduces the number of infective phage particles.
Concentration of KVP40 (Myoviridae) and ϕH20 (Siphoviridae) was reduced post 48 h of incubation in cell-free spent supernatant (Nt) compared with incubation in growth medium (control, Nc). An asterisk indicates a significant reduction of infective phages compared with cell-free spent supernatants from the ΔH20 cultures (ANOVA and Sidak’s multiple comparison test, α = 0.05). Error bars represent standard error of the mean (N = 3).