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. 2020 Jun 19;11:3116. doi: 10.1038/s41467-020-16922-1

Fig. 2. Talin domains bind to vinculin differently in response to the actomyosin force.

Fig. 2

a Time lapses showing the recruitment of Vh to talin R1–R2–R3-coated disks in the absence (top) or presence of actomyosin (Vh is shown on the middle and actin on the bottom panel). This experiment was repeated five times independently with the same results. b–d Kymographs of EGFP-Vh (top) and actin (bottom) along a cross-section of a disk coated with talin R1–R2–R3 (b) or R11 (c) or R7–R8 (d) in the absence (left) or presence (right) of actomyosin. Conditions: 100 nM EGFP-Vh, 2.4 µM actin (2% Alexa594-labeled), 50 nM myosin, and 1 µM talin during the coating step. The images are color coded using the fire LUT of ImageJ. Scale bar in time lapses = 10 µm. In kymographs, horizontal bar = 500 s, vertical bar = 5 µm. e–g Kinetics of the mean fluorescence of EGFP-Vh corresponding to the conditions described in (b–d). Data are mean ± SD. e n = 80 (−actomyosin), n = 60 disks (+actomyosin). f n = 60 (−actomyosin), n = 75 disks (+actomyosin). g n = 80 (−actomyosin), n = 76 disks (+actomyosin). Source data are provided as a Source data file. See Supplementary Movie 1, Supplementary Movie 2, and Supplementary Movie 3.