Figure 1.

Analysis of conditional ablation/inactivation of Pten, Trp53, Cdh1, and Rb1 induced by Amhr2^Cre/+ mice in mouse ovary. (A) Genotyping PCR of tail DNA to detect the Amhr2^Cre/+ transgene (three representative mice per each genotype and heterozygous, f/+). (B) QPCR analysis of Trp53 normalized to Rpl19 in the ovary of control and Trp53^d/d mice. Immunolocalization of (C) PTEN in the ovary of control and Pten^d/d mice; (D) CDH1 in the ovary of control and Cdh1^d/d mice; and (E) SV40 T antigen in the ovary of control, TgK19GT₁₂₁, Pten^d/d Trp53^d/d TgK19GT₁₂₁, and Pten^d/d Trp53^d/d Cdh1^d/d TgK19GT₁₂₁ mice. Black arrows show positive immunostaining.