Figure 3.

Mechanical strength and swelling ratio of Sac/EGF-HF (a) Mechanical Strength of Sac/EGF-HF (b) Swelling ratio of Sac/EGF-HF (a) Tensile strength was done by placing hydrogel films on the sample holder with two holding grips and pulled by the top clip at a rate of 0.5 mm/s. Then, hydrogel films were analyzed by Textechnofavigraph (Monchengladbach, Germany) (b) Swelling ratio of Sac/EGF-HF was measured by the gravimetric method in PBS solution (pH 7.4) for 48 h. Each value represents the mean ± SEM of three experiments. *P < 0.05, compared to Sac-HF. Sca/EGF-HF: Sacran/epidermal growth factor-hydrogel film, PBS: Phosphate buffered saline