Fig. 2.

MS2 spectra of two distinct peptides carrying N45 glycosylation site along with L/H at position 48. (A) Three peptides are generated as a result of Chymotrypsin (Chy.) and GluC (Glu.) digestion of CD16a. Peptides 2 and 3 carry both the N45 glycosylation site (red N) and L/H at position 48 (blue L and H) and are produced by the missed GluC cleavage at E46. (B) MS2 spectra for peptide 2 carrying a hybrid type N-glycan at N45 from the NK cell donor. Hybrid type N-glycan on peptide 3 from NK cell (C) and monocyte (D).