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. Author manuscript; available in PMC: 2020 Jun 20.
Published in final edited form as: Reproduction. 2019 Feb;157(2):179–188. doi: 10.1530/REP-18-0424

Figure 4.

Figure 4

(A) The predicted results of the interaction between MALAT1 and miR200s by RNAhybrid (https://bibiserv.cebitec.unibielefeld.de/rnahybrid). (Red: MALAT1. Green: each member of miR200s) (MALAT1 and miR200a, mfe: −22.6 kcal/mol; MALAT1 and miR200b, mfe: −26.8 kcal/mol; MALAT1 and miR200c, mfe: −30.1 kcal/mol; MALAT1 and miR141, mfe: −24.2 kcal/mol; MALAT1 and miR429, mfe: −21.0 kcal/mol.); (B and C) Ishikawa cells were transfected with miR200c mimic or miRNA Negative Control (miRNA NC) and were treated with or without E2 (10−8 mol/L) for 24 h. The expression levels of miR200c and MALAT1 were analyzed by qRT-PCR. (D) Expression levels of EMT-associated markers detected by Western blot after the same treatment for 48 h. (E) The function of miR200c mimic and/or E2 affects the ability of migration (24 h) and invasion (48 h) in Ishikawa cells. Data were evaluated by one-way ANOVA analysis (*P < 0.05, **P < 0.01, ***P < 0.001). Photographs were taken at magnifications of 200×.