(A) The effect of miR200c inhibitor on protein expression levels of down-stream EMT-associated markers for 48 h analyzed by Western blot. (B, C and D) Ishikawa cells were co-transfected with si-MALAT1(−2) or si-NC, and miR200c inhibitor or inhibitor Negative Control (inhibitor NC); (B) Expression levels of Zeb1, Zeb2, E-cadherin and Vimentin after treatment for 48 h analyzed by Western blot; (C) Expression level of MALAT1 after treatment for 24 h analyzed by qRT-PCR; (D) The treatment influences the ability of migration and invasion; (E) Estradiol promotes epithelial– mesenchymal transition in endometriosis via MALAT1/miR200s sponge function. E2-activated ERβ may promote the expression of MALAT1 and mediate EMT in endometriosis. Data were evaluated by one-way ANOVA analysis (*P < 0.05, **P < 0.01, ***P < 0.001). Photographs were taken at magnifications of 200×.