| Component | Volume (μl) | Final concentration |
|---|---|---|
| H2O | 9 | |
| gDNA (100 ng, 100 ng/μl, from Step 5 of this box | 1 | 4 ng/μl |
| Custom primer F (10 μM) | 1.25 | 0.5 μM |
| Custom primer R (10 μM) | 1.25 | 0.5 μM |
| 2x Q5 Master Mix | 12.5 | 1x |
| Total | 25 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.