| Step | Temperature (C) | Time (sec) | Cycles |
|---|---|---|---|
| Denaturation | 98 | 30 | 1 |
| Denaturation | 98 | 10 | 30 |
| Annealing | Primer dependent | 20 | 30 |
| Extension | 72 | 20 | 30 |
| Extension | 72 | 120 | 1 |
| Hold | 4 | 1 |
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