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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Nat Protoc. 2020 Apr 20;15(5):1775–1799. doi: 10.1038/s41596-020-0309-5
Volume Reagent Stock conc. Final conc.
146.5 mL MilliQ water - -
10 mL HEPES-KOH pH 7.5 1 M 50 mM
5.6 mL NaCl 5 M 140 mM
400 μL EDTA pH 8.0 0.5 M 1 mM
25 mL Glycerol 80 % (vol/vol) 10 % (vol/vol)
10 mL Igepal CA-630 10 % (vol/vol) 0.5 % (vol/vol)
2.5 mL Triton X-100 20 % (vol/vol) 0.25 % (vol/vol)