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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Nat Protoc. 2020 Apr 20;15(5):1775–1799. doi: 10.1038/s41596-020-0309-5
Volume Reagent Stock conc. Final conc.
184 mL MilliQ water - -
25 mL HEPES-KOH pH 7.5 1 M 50 mM
250 mL LiCl 1 M 500 mM
1 mL EDTA pH 8.0 0.5 M 1 mM
5 mL Igepal CA-630 undiluted 1 % (vol/vol)
35 mL Na-deoxycholate 10 % (wt/vol) 0.7 % (vol/vol)