| Volume | Reagent | Stock conc. | Final conc. |
|---|---|---|---|
| 95 mL | MilliQ water | - | - |
| 2 mL | Tris-HCl pH 8.0 | 1 M | 20 mM |
| 3 mL | NaCl | 5 M | 150 mM |
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