| Volume | Reagent | Stock conc. | Final conc. |
|---|---|---|---|
| 41.5 mL | MilliQ water | - | - |
| 2.5 mL | Tris-HCl pH 8.0 | 1 M | 50 mM |
| 1 mL | EDTA pH 8.0 | 0.5 M | 10 mM |
| 5 mL | SDS | 10 % (wt/vol) | 1 % (vol/vol) |
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