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. 2020 Jun 16;13:5643–5655. doi: 10.2147/OTT.S254662

Figure 3.

Figure 3

Long intergenic non-protein coding RNA 1224 (LINC01224) functions as a molecular sponge of microRNA-485-5p (miR-485-5p) in epithelial ovarian cancer (EOC) cells. (A) Subcellular fractionation and qRT-PCR indicated that LINC01224 was mainly located in the cytoplasm of Caov-3 and OVCAR3 cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and U6 small nuclear RNA acted as the controls to evaluate the fractioning efficiency. (B) The schematic illustration of wild-type (wt) and mutant (mut) binding sites between miR-485-5p and LINC01224. (C) qRT-PCR results revealed the expression of miR-485-5p in miR-485-5p mimic or miRNA mimic negative control (miR-NC)-transfected Caov-3 and OVCAR3 cells. (D) Relative luciferase activity was measured in Caov-3 and OVCAR3 cells co-transfected with wt-LINC01224 or mut-LINC01224 reporter plasmid and miR-485-5p mimic or miR-NC. (E) The interaction between miR-485-5p and LINC01224 was evaluated in Caov-3 and OVCAR3 cells using the RNA immunoprecipitation (RIP) assay. Immunoglobulin G (IgG) acted as the negative control. (F) The expression of miR-485-5p was measured using qRT-PCR in EOC tissues and corresponding adjacent normal tissues. (G) Pearson’s correlation coefficient analysis identified an inverse correlation between miR-485-5p and LINC01224 expression in EOC tissues (r = −0.5315, P < 0.0001). (H) qRT-PCR results uncovered miR-485-5p expression in Caov-3 and OVCAR3 cells after small interfering RNA targeting LINC01224 (si-LINC01224) or negative control small interfering RNA (si-NC) transfection. **P < 0.01.