Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 16;13:5643–5655. doi: 10.2147/OTT.S254662

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Xing et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Long intergenic non-protein coding RNA 1224 (LINC01224) drives the malignant progression of epithelial ovarian cancer (EOC) cells by modulating the microRNA-485-5p (miR-485-5p)/p21‑activated kinase 4 (PAK4) axis. (A and B) qRT-PCR and Western blotting were conducted to measure the expression levels of PAK4 mRNA and protein in Caov-3 and OVCAR3 cells after small interfering RNA targeting LINC01224 (si-LINC01224) or negative control small interfering RNA (si-NC) transfection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a loading control. (C) The knockdown efficiency of miR-485-5p inhibitor in Caov-3 and OVCAR3 cells was determined by qRT-PCR. Negative control (NC) inhibitor served as the control. (D and E) si-LINC01224, together with miR-485-5p inhibitor or NC inhibitor, was transfected into Caov-3 and OVCAR3 cells. The transfected cells were collected, and the mRNA and protein levels of PAK4 via qRT-PCR and Western blotting were measured. (F) The efficiency of pCDNA3.1-PAK4 (pc-PAK4) transfection was detected by Western blotting. The empty pcDNA3.1 vector served as the control. (G and H) LINC01224-depleted Caov-3 and OVCAR3 cells were co-transfected with miR-485-5p inhibitor or pc-PAK4. The CCK-8 assay and flow cytometry analysis were used to assess cell proliferation and apoptosis, respectively. PI, propidium iodide. (I and J) The migration and invasion of the cells mentioned above were detected via cell migration and invasion assays. *P < 0.05, **P < 0.01.