Table 1.
Primer sequences used for RT-PCR analysis.
| Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
| Mouse β-ACTIN | TCCATCATGAAGTGTGACGT | GAGCAATGATCTTGATCTTCAT |
| Mouse Muc5ac | CTACTGACTGCACCAACACAT | GTGCAGTCCCCATGTACTGT |
| Mouse Muc5b | TAGCATGAGCGCCTTACACC | CACGACGCAGTTGGATGTTG |
| Mouse IL-6 | TGTGCAATGGCAATTCTGAT | CTCTGAAGGACTCTGGCTTTG |
| Mouse TNF-α | GGCGGTGCCTATGTCTCA | TCCACTTGGTGGTTTGTGA |
| Mouse CD86 | TCTGCCGTGCCCATTTACAA | TGTGCCCAAATAGTGCTCGT |
| Mouse IL-12 | GGGGTGTAACCAGAAAG | CATGAGGAATTGTAATAGCG |
| Mouse IL-10 | ACCTGGTAGAAGTGATGCC | CACCTTGGTCTTGGAGCT |
| Mouse CD206 | AGGCTGATTACGAGCAGTGG | CCATCACTCCAGGTGAACCC |
| Mouse TGF-β | AAGTGGGCCGAGAACTGG | CCTCCTTCACGCTGACAATC |
| Mouse Egfr | GAAGAAGTGCCCCCGAAACT | TCGTAGTAGTCAGGCCCACA |
| Human β-ACTIN | CCAACCGCGAGAAGATGA | CCAGAGGCGTACAGGGATAG |
| Human MUC5AC | AGCCGGGAACCTACTACTCG | TGGTCATAGGCTTCGTGCAG |
| Human MUC5B | CAAGTCCGCATCAACACGAC | TGCTGAGTACTTGGACGCTC |
| Human IL-6 | CCTGAACCTTCCAAAGATGGC | TTCACCAGGCAAGTCTCCTCA |
| Human TNF-α | AGCCCATGTTGTAGCAAACC | TGAGGTACAGGCCCTCTGAT |
| Human CD86 | CGACGTTTCCATCAGCTTGTC | CGCGTCTTGTCAGTTTCCAG |
| Human IL-12 | CACAAAGGAGGCGAGGTTCT | TTTGGGTTCTTTCTGGTCCTT |
| Human IL-10 | CCAGACATCAAGGCGCATGT | GATGCCTTTCTCTTGGAGCTTATT |
| Human CD206 | GATTGCAGGGGGCTTATGGG | CGGACATTTGGGTTCGGGAG |
| Human TGF-β | GCAACAATTCCTGGCGATACC | ATTTCCCCTCCACGGCTCAA |
| Human EGFR | CCGCAAAGTGTGTAACGGAA | CCACTGATGGAGGTGCAGTT |