Uncompensated contribution of LCI1 (C, D), LCIB (E, F), and LCIA (G, H) in a wild-type background at pH 6 (open squares), pH 7.3 (closed squares) and pH 9 (red circles) plotted against the calculated CO2 concentration (left) and HCO3− concentration (right). The CO2 and HCO3− concentration ranges covered are 0–250 μM and 0–1200 μM, respectively. A and B are the original wild-type O2 evolution rates. Note that the Y-axis scales on the original O2 evolution plots (A, B) are different than those on the uncompensated contribution plots (C–H). Uncompensated contributions in wild type were calculated by subtracting O2 evolution rates of the single mutant from wild type strain (LCI1 in wild type = 21gr-lci1; LCIB in wild type = 21gr-pmp1; LCIA in wild type = 21gr-lcia).