Fig. 1.

A siRNA screen targeting nuclear enzymes identifies genes that mediate cisplatin resistance in SCLC. a Primary screen format: H128 cells were transfected with a siRNA library biased towards targeting nuclear enzymes. 48 hours post-transfection, cells were treated with or without 10 μM cisplatin for 72 hours prior to measuring cell viability. b Primary screen results: the normalized cell viability was plotted against the strictly standardized mean difference (SSMD) for each gene targeted by the library. Normalized viability was calculated as the log₂ ratio of treated versus untreated cell viability relative to the non-targeting (NT) siRNA control. c Summary of most enriched categories from Gene Ontology (GO) analysis of cisplatin sensitization hits (n = 118). The top 10 significant processes (p ≤ 0.01) are shown, sorted by the percent associated genes found.