Table 4.

Differentially expressed pharmacologically relevant genes (RKs boldfaced) in intact vs cultured human DRGs with | SSMD | > 2, partitioned by mean TPM in condition of higher expression. The number of genes in each column is shown in parentheses. Genes known to be associated with pain from GWAS / functional association databases and the literature are underlined. Typically, smaller TPM brackets have higher technical variance.

GPCRs		Ion channels		Kinases
Up in native DRG (85)	Up in cultured DRG (7)	Up in native DRG (109)	Up in cultured DRG (7)	Up in native DRG (105)	Up in cultured DRG (22)
> 50 TPM
ADGRA2, ADGRB3, ADGRL1, ADGRL3, ADGRV1, GABBR1, GPR17, LGR5, LPAR6, TACR2	GPR33, P2RY11	ANO5, AQP1, CACNA1A, CACNA1B, CACNA2D1, CACNA2D3, CACNB1, CACNB3, CLCN2, CLCN6, GRIK2, GRIN1, ITPR3, KCNQ2, P2RX3, PKD2L2, RYR1, RYR2, RYR3, SCN10A, SCN11A, SCN1B, SCN4B, SCN8A, SCN9A, TPCN1, TRPV1, ZACN	CLIC1, CLIC4, P2RX4, ANO6	BCR, BRAF, BRD2, BRSK2, CAMK1D, CAMK2A, CAMK2B, CAMK2G, CAMKK1, CDK10, CDK9, CDKL1, CLK1, CLK2, CLK4, CSNK1G2, DCLK2, ERBB3, FER, FGFR1, KALRN, KSR1, LIMK1, MAP2K5, MAP3K5, MAP3K6, MAPK12, MARK1, MARK2, MAST1, MAST2, MAST3, NEK1, NRBP2, NPR2, NTRK1, NTRK3, PINK1, PRKACA, PRKCA, PRKCZ, RET, ROCK2, SPEG, SRPK2, TAF1, TGFBR2, TLK2, TRRAP, TYRO3, ULK2, ULK3, WNK1	ABL2, AXL, CDK2, CDK4, DAPK3, ILK, MAPKAPK2, MYLK, NRBP1, PDGFRB, PTK7, TRIO
> 25 TPM
ADGRB1, ADGRG2, CELSR2, GABBR2, GPR18, GPR183, MRGPRE	ADGRF5, ADGRL4	ANO3, ANO8, AQP11, CACNA1C, CACNA1D, CHRNA7, CHRNE, CLCN5, GABRA2, GABRB3, GRIA4, GRIK3, GRIK5, HCN2, MCOLN3, SCN1A, TRPM2	KCNN4	BMPR2, DCLK1, EGFR, HIPK2, HSPB8, LRRK1, LRRK2, MAPK11, MST1, NTRK2, PAK3, PRKACB, PRKCB, PRKCE, PRKCI, SLK, TSSK3, TSSK4, TTBK2, TTN, ULK1	DYRK4, FLT1, MAPKAPK3, MELK
> 12.5 TPM
CCR10, CHRM5, CXCR6, GNRHR, GPR146, GPR171, GPR37L1, GPR62, GPRC5B, GRM7, HTR2B, OR10AD1, OR2A1, OR2A42, OR52B6, P2RY12, P2RY14, SSTR1, TAS1R3, TAS2R40	CXCR4	ANO4, ASIC1, CACNB2, CACNB4, CACNG7, CHRNB1, CLCN4, GABRG2, GJA9, GLRB, GRIA2, GRIK1, GRIN3B, KCNC1, KCND1, KCNH2, KCNQ5, KCNT2, LRRC8B, NALCN, P2RX5, P2RX6, SCNN1D	AQP9	ACVR2A, ADCK1, BRD3, CDKL2, DAPK2, DSTYK, EPHA5, EPHA6, EPHB3, EPHB6, MAK, MAP2K6, MUSK, MYT1, OBSCN, POMK, PRKX, TNK1, TTBK1, WNK2	IRAK2, PLK1, TRIB3, TTK
> 6.3 TPM
AVPR2, CELSR3, F2RL2, FZD2, GPER1, GPR158, GPR162, GPR182, GPR82, GPR87, GRM4, LPAR3, OPRM1, OR1K1, OR2A7, OR3A3, OR51I2, OR51J1, OXER1, PTGER4, TAS2R39, TSHR	-	ANO2, CFTR, CHRNG, CLIC3, GJA4, GJB7, GJC3, GRIN2B, HCN1, KCNA2, KCNH7, KCNH8, KCNK12, KCNN2, KCNS1, LRRC8D, SCN3B	-	EPHA10, FLT3, HIPK4, LMTK2, NEK5, PNCK, STKLD1	NEK2
> 3.1 TPM
GPR149, GPR45, GRM3, GRM6, MRGPRF, OR11L1, OR13J1, OR5C1, OR6F1, OR6V1, PITPNM3	GPR176, F2R	AQP12B, GJB3, GRIN3A, KCNB2, KCNH1, KCNH3, KCNH6, KCNJ3, KCNK1, KCNK15, TRPC5	-	KSR2, PRKAA2	DYRK3
<= 3.1 TPM
ADRA2C, CCKBR, CXCR3, GPR135, GPR179, GPR26, GRM8, HTR5A, HTR6, OPRL1, OR1Q1, OR2AE1, OR5AS1, SSTR2, TBXA2R	-	GLRA3, HCN3, KCNK10, KCNH5, KCNJ10, KCNJ12, KCNJ5, KCNJ9, KCNQ4, KCNV1, LRRC8E, SCN2B, TRPC3	KCNJ8	FRK, PRKCQ	-