Table 5.

Differentially expressed pharmacologically relevant genes (RKs boldfaced) in intact vs cultured mouse DRGs, with | SSMD | > 2 partitioned by mean TPM in condition of higher expression. The number of genes in each column is shown in parentheses. Typically, smaller TPM brackets have higher technical variance.

GPCRs		Ion channels		Kinases
Up in native DRG (95)	Up in cultured DRG (20)	Up in native DRG (122)	Up in cultured DRG (14)	Up in native DRG (70)	Up in cultured DRG (66)
> 50 TPM
Adora1, F2rl2, Gabbr1, Gabbr2, Gpr137, Lpar3, Mrgprd	Adgre5, Mrgprf, Smo	Ano3, Asic1, Asic2, Asic3, Cacna1a, Cacnb1, Cacnb3, Cacng7, Clcn2, Clcn6, Gabrg2, Glrb, Grik1, Grik5, Grin1, Hcn2, Htr3a, Kcna2, Kcnc4, Kcnd1, Kcnk1, Kcns1, Kcns3, Kcnt1, Mcoln1, Scn10a, Scn11a, Scn1b, Scn4b, Trpc3, Trpm4, Trpv1	Clic1, Clic4, Gja1, Kcnn4, Lrrc8c	Akt3, Brsk1, Brsk2, Camk2a, Camk2b, Camk2g, Cdk10, Cdk5, Cdkl2, Kit, Limk1, Limk2, Map2k4, Mapk11, Mapk12, Mast1, Nek1, Nek7, Npr2, Nrbp2, Ntrk1, Ntrk2, Phkg2, Pim3, Pink1, Prkaca, Prkacb, Prkca, Prkcb, Prkcd, Prkce, Prkcq, Prkcz	Acvr1, Aurka, Aurkb, Axl, Bckdk, Bmpr1a, Cdk4, Ddr1, Erbb3, Ilk, Mapkapk2, Mark3, Pak2, Pbk, Pdgfrb, Plk1, Plk2, Riok3, Rock2, Ryk, Srm
> 25 TPM
Adgrb1, Cysltr2, Gpr45, Gprc5c, Grm7, Htr1d, P2ry1, Ptgdr, Pth1r	Adgra2, Fzd1, Fzd2	Ano8, Cacna1h, Cacna2d3, Chrna6, Chrnb2, Gabrb3, Gria4, Hcn3, Kcnb1, Kcnc1, Kcnc3, Kcnj12, Kcnn1, Kcnq4, Tpcn1, Trpa1	Gjc1, Kcnk5, Pkd2	Acvr1b, Camk1g, Dclk3, Mark1, Myt1, Ntrk3, Pim2, Pnck, Ttbk1	Abl1, Bub1, Cdk2, Cdk7, Ddr2, Ephb2, Ephb3, Erbb2, Map3k3, Melk, Met, Nek2, Peak1, Pkd2, Pkn2, Plk4, Prag1, Ripk1, Ripk3, Tec, Tgfbr1, Tgfbr2
> 12.5 TPM
Adgrb2, Adgrg2, Adra2c, Agtr1a, Cckar, Cxcr4, Gpr161, Gpr162, Gpr27, Gpr35, Grm4, Mrgpra2a, Mrgpra2b, Mrgprx1, Npy2r, Opn3, Oprl1, Ptger1, Ptgfr	Ackr3, Fzd7, S1pr2	Cacna2d2, Cacng5, Chrna3, Chrna7, Gabra1, Gabra2, Grik4, Htr3b, Kcnb2, Kcng2, Kcnh1, Kcnh6, Kcnk2, Kcnk3, Kcnk4, Kcnn2, P2rx6, Scn1a, Scn2b, Trpm2	Clcn5	Camk1d, Camkk1, Dapk2, Flt3, Lmtk3, Map3k5, Pdk1, Wnk2	Cdc7, Cdk6, Ephb4, Fer, Haspin, Irak4, Jak2, Lyn, Mastl, Mlkl, Pask, Riok1, Rock1, Scyl3, Trib3, Ttk, Vrk1, Vrk2, Wee1
> 6.3 TPM
Adora2a, Chrm4, Cnr1, Gpr135, Gpr139, Gpr146, Gpr149, Gpr157, Gpr37, Gpr61, Gpr75, Grm8, Hcrtr1, Htr1b, Htr7, Lgr5, Lpar5, Prokr1, Ptger3, Ptger4, Sstr2	C3ar1, C5ar1, Celsr1, Cmklr1, Fzd6, Lpar6	Aqp11, Cacna1d, Cacng2, Cacng4, Chrnb3, Chrnb4, Gabra3, Gabrg1, Gjb6, Gria1, Grin3a, Hcn4, Kcna4, Kcnc2, Kcng4, Kcnh7, Kcnj11, Kcnj3, Kcnj4, Kcnq3, Kcnq5, Kcnu1, Kcnv1, Nalcn, P2rx2, P2rx5, Ryr2, Trpc6, Trpm8	-	Amhr2, Cdkl1, Mst1r, Prkaa2, Tie1	Ikbke, Map3k2
> 3.1 TPM
Chrm2, Crhr2, Gpr156, Gpr160, Gpr22, Gpr3, Gpr68, Hcrtr2, Hrh3, Htr1a, Htr4, Nmur1, Ntsr2, Pitpnm3	Adra1b, F2rl1, P2ry6	Cacna1i, Gria2, Kcnk18, Scn5a	Gjb5, Lrrc8e	Brdt, Camk4, Epha10, Ksr2, Npr1, Tek	Ror1
<= 3.1 TPM
Adrb1, Ccr10, Ccr2, Chrm1, Crhr1, Galr1, Gpr150, Gpr174, Gpr179, Gpr182, Gpr21, Gpr26, Gpr62, Gpr83, Gpr88, Hcar1, Hrh2, Htr5a, Lhcgr, Mas1, Oprd1, Oprk1, Ptafr, Ptger2, Rho	Ccr5, Gpr84	Aqp4, Best1, Cacna1e, Cacng3, Chrna10, Chrna4, Clic5, Gabrb1, Gabrb2, Gjd2, Kcnd2, Kcng1, Kcng3, Kcnh5, Kcnj13, Kcnj14, Kcnj16, Kcnj2, Kcnj8, Kcnj9, Kcnn3, Kcns2, Scnn1a	Chrna1, Gjb4, Itpr2	Bmx, Cdkl4, Epha6, Epha8, Ephb1, Insrr, Itk, Nek11, Sbk3	Frk