KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-BRD4 | Bethyl laboratories | Cat# A301–985A; RRID:AB_1576498 |
| Rabbit monoclonal anti- BRD2 | Cell Signaling Technology | Cat# 5848; RRID:AB_10835146 |
| Rabbit monoclonal anti- BRD7 | Cell Signaling Technology | Cat# 14910; |
| Mouse monoclonal anti-H3K4me3 | Abcam | Cat# ab1012; RRID: AB_442796 |
| Rabbit Anti-Histone H3, Trimethyl (Lys27) Monoclonal Antibody, | Cell Signaling Technology | Cat# 9733, RRID:AB_2616029 |
| Rabbit monoclonal anti- RB | Cell Signaling Technology | Cat# 9313; RRID:AB_1904119 |
| Rabbit monoclonal anti- CCND1 | Cell Signaling Technology | Cat# 2978; RRID:AB_2259616 |
| Rabbit monoclonal anti- CDK4 | Cell Signaling Technology | Cat# 12790; RRID:AB_2631166 |
| Mouse monoclonal anti-beta-Actin | Sigma-Aldrich | Cat# A2228; RRID: AB_476697 |
| Rabbit polyclonal anti-H3K27Ac | Abcam | Cat# ab4729; RRID: AB_2118291 |
| Mouse monoclonal anti- E2F1 | Cell Signaling Technology | Cat# 3742; RRID:AB_2096936 |
| Rabbit monoclonal anti- Phospho-Rb-Ser780 | Cell Signaling Technology | Cat# 3590; AB_2177182 |
| Rabbit monoclonal anti- Phospho-Rb-Ser807/811 | Cell Signaling Technology | Cat# 9308; RRID:AB_331472 |
| Rabbit monoclonal anti- Phospho-Rb-Ser807/811 | Cell Signaling Technology | Cat# 8516; RRID:AB_11178658 |
| Rabbit monoclonal anti- cleaved Caspase-3 | Cell Signaling Technology | Cat# 9664; RRID:AB_2070042 |
| Rabbit polyclonal Phospho-Stat-3 (tyr705) antibody | Cell Signaling Technology | Cat# 9131, RRID:AB_331586 |
| Mouse monoclonal anti- BrdU | Roche | Cat# 11170376001; RRID:AB_514483 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| JQ1 | Jun Qi (Dana-Farber Cancer Institute) | N/A |
| Palbociclib | MedChem Express | HY-50767A |
| ABT-199 | Selleckchem | S8048 |
| ABT263 | Selleckchem | S1001 |
| INC424 | InCyte | N/A |
| Dasatinib | Selleckchem | S1021 |
| TP-0903 | Selleckchem | S7846 |
| Paclitaxel | Dana-Farber Cancer Institute Research Pharmacy | N/A |
| Doxorubicin | Dana-Farber Cancer Institute Research Pharmacy | N/A |
| Vincristine | Sigma-Aldrich | V8879 |
| Gemcitabine | Sigma-Aldrich | G6423 |
| dBET series (dBET1 - dBET6) | Dennis Buckley (Dana-Farber Cancer Institute) | N/A |
| Flavopiridol | Selleckchem | S1230 |
| Barasertib | Selleckchem | S1147 |
| Target Selective Inhibitor Library | Selleckchem | L3500 |
| Anti-cancer Compound Library | Selleckchem | L3000 |
| Critical Commercial Assays | ||
| ThruPLEX DNA-seq 48S Kit | RUBICON | R400427 |
| Experimental Models: Cell Lines | ||
| SUM159 cell line | Steve Ethier (University of Michigan) | N/A |
| SUM149 cell line | Steve Ethier (University of Michigan) | N/A |
| SUM159R cell line | Shu et al., 2016 | N/A |
| SUM149R cell line | Shu et al., 2016 | N/A |
| SUM159 cell line, BRD2 KO | This paper | N/A |
| SUM159 cell line, BRD7 KO | This paper | N/A |
| SUM159 cell line, CDK4 KO | This paper | N/A |
| CAL-51 cell line | DSMZ | ACC 302 |
| CAL-120 cell line | DSMZ | ACC 459 |
| HCC38 cell line | ATCC | CRL-2314 |
| HCC3153 cell line | Adi Gazdar/LBL Joe Gray | N/A |
| PMC42 cell line | R.Whitehead | N/A |
| MDA-MB-436 cell line | ATCC | HTB-130 |
| MDA-MB-231 cell line | ATCC | HTB-26 |
| Hs578T cell line | ATCC | HTB-126 |
| Oligonucleotides - sgRNA targeting sequences | ||
| BRD2–1: CTTCTCATCGTAACTCATG | This paper | N/A |
| BRD2–2: CCACGCAAAGACTTGCCTG | This paper | N/A |
| BRD7–1: CGGGCAGCTCGGGGCACGA | This paper | N/A |
| BRD7–2: CCTACAATGGGATCCACAG | This paper | N/A |
| CDK4–1: CCAGAGGATGACTGGCCTC | This paper | N/A |
| CDK4–2: CCTCACGAACTGTGCTGAT | This paper | N/A |
| Recombinant DNA | ||
| CRISPR-V2 | Sanjana et al, 2014 | Addgene Cat# 52961 |
| Plv(Exp)-Bsd-TRE-CDK4 | This paper | Vector builder |
| Deposited Data | ||
| All raw genomic data | GEO | GSE131102 |