REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb antibody	Cell Signaling Technology	Cat# 9106S; RRID: AB_331768
p44/42 MAPK (Erk1/2) Antibody	Cell Signaling Technology	Cat# 9102S; RRID: AB_330744
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb	Cell Signaling Technology	Cat# 9234S; RRID: AB_2269803
Rabbit Anti-p70 S6 Kinase Monoclonal Antibody	Cell Signaling Technology	Cat# 2708S; RRID: AB_390722
c-MYC antibody	Cell Signaling Technology	Cat#9402; RRID: AB_2151827
Anti-mouse IgG, HRP-linked Antibody	Cell Signaling Technology	Cat#7076; RRID: AB_330924
Anti-rabbit IgG, HRP-linked Antibody	Cell Signaling Technology	Cat#7074; RRID: AB_2099233
Phospho-PFAS T619 Rabbit antibody	Cell Signaling Technology	In development
PFAS Antibody	Bethyl Laboratories	A304–218A; RRID: AB_2620417
ATIC Antibody	Bethyl Laboratories	A304–272A; RRID: AB_2620468
PPAT Antibody	Proteintech Group Inc	15401–1-AP; RRID: AB_2166532
GART Antibody	Proteintech Group Inc	13659–1-AP; RRID: AB_2107937
ADSL Antibody	Proteintech Group Inc	15264–1-AP; RRID: AB_2225668
ADSS Antibody	Proteintech Group Inc	16373–1-AP; RRID: AB_2225676
PAICS Antibody	Proteintech Group Inc	12967–1-AP; RRID: AB_10638449
IMPDH1 Antibody	Proteintech Group Inc	Cat#22092–1-AP
IMDPH2 Antibody	Proteintech Group Inc	12948–1-AP; RRID: AB_2127351
GMPS Antibody	Proteintech Group Inc	16376–1-AP; RRID: AB_2232412
β-actin Antibody	Sigma-Aldrich	A5316; RRID: AB_476743
Anti-FLAG M2	Sigma-Aldrich	F1804; RRID: AB_262044
Bacterial and Virus Strains
NEB Stable Competent E. coli	NEB	C3040
DH5 alpha	Fisher Scientific	18258012
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
U0126 MEK inhibitor	Tocris Biosciences	1144
AZD6244	Selleckchem	S1008
SCH772984	Selleckchem	S7101
EGF (human recombinant)	Life Technologies (Gibco)	PHG0311
L-glutamine	Corning	MT25005Cl
13C2-15N-glycine	Sigma-Aldrich	489522
13C4-15N-aspartic acid	Sigma-Aldrich	607835–250MG
L-glutamine-(amide-15N)	Sigma-Aldrich	490024
thiazolyl Blue Tetrazolium Bromide	Sigma-Aldrich	M5655
DMSO	Sigma-Aldrich	D2650–100ML
2-mercaptoethanol	Sigma-Aldrich	M6250–100ML
Hypoxanthine	Sigma-Aldrich	H9636–1G
Inosine	Sigma-Aldrich	I4125–1G
1,2-3H-2-deoxy-D-Glucose	Perkin-Elmer	NET328A250UC
U-14C-glycine	Perkin-Elmer	NEC276E050UC
U-14C-glucose	Perkin-Elmer	NEC042V250UC
3-14C-serine	Perkin-Elmer	NEC853050UC
3H-hypoxanthine	Perkin-Elmer	NET177001MC
[γ−32P]-ATP	Perkin-Elmer	BLU002A100UC
14C-formate	American Radiolabeled Chemicals	ARC 0163A
Polyethylenimine (PEI)	Polysciences	23966–2
Opti-MEM media	Thermo Fisher	31985062
Glutamine-free DMEM	Gibco	11960–044
Geneticin Selective Antibiotic	Fisher Scientific	10131035
Lipofectamine 3000 Transfection reagent	Invitrogen	L3000008
Glucose-free DMEM	Invitrogen	11966025
Lipofectamine RNAi max	Invitrogen	13778150
DpnI
SuperSignal™ West Femto Chemiluminescent	Thermo Fisher scientific	PI34096
SuperSignal™ West Pico PLUS	Thermo Fisher scientific	PI34580
Protein A/G AGAROSE 3ML/PK	Thermo Scientific	20421
Recombinant Protein - MAPK3 (ERK1)	MRC-PPU	2 – 379
Recombinant Protein - MAPK1 (ERK2)	MRC-PPU	2 – 360
DMEM	Corning CellGro	10–017-CV
Glycine (and serine)-free DMEM	US Biologicals	D9800–16
Fetal bovine serum (FBS)	Sigma-Aldrich	F2442
Dialyzed FBS (dFBS)	Sigma-Aldrich	F0392
Microcystin-LR	Fisher Scientific	ALX350012C500
Protease inhibitor cocktail	Sigma-Aldrich	P8340
Critical Commercial Assays
RNeasy Mini Kit	QIAZEN	74104
HiSpeed Plasmid Maxi Kit (25)	QIAZEN	12663
AllPrep DNA/RNA Mini Kit	QIAZEN	80204
CellTiter-Glo Luminescent Cell Viability Assay	Promega	G7573
KOD Extreme Hot Start DNA polymerase kit	Merck Millipore	71975–3
Deposited Data
A study of Ali, Sahu et al_Unprocessed file	Mendeley Data	http://dx.doi.org/10.17632/px6zm7ms9r.3
Experimental Models: Cell Lines
HEK293E cells	John Blenis lab (Weill Cornell Medicine)	N/A
HeLa cells	ATCC	CCL-2
A549 cells	ATCC	CCL-185
SKMEL-28 cells	ATCC	HTB-72
Panc1 cells	ATCC	CRL-1469
Mouse Embryonic Fibroblasts (MEFs)	David Kwiatkowski (Brigham and Women’s Hospital)	N/A
Experimental Models: Organisms/Strains
Oligonucleotides
See Table S3 for list of primers used in this study	Integrated DNA Technologies
siRNA targeting siMYC	Dharmacon	L-003282-02-0005
Non-targeting siRNA pool	Dharmacon	D-001810-10-20
Recombinant DNA
PFAS Human Tagged ORF Clone	Origene	RC217795
pCMV6-Entry Tagged Cloning Vector	Origene	PS100001
pLenti-III-PGK vector	Applied Biological Materials	G305
pCMV6-PFAS-S162A	This study	NA
pCMV6-PFAS-S215A	This study	NA
pCMV6-PFAS-T619A	This study	NA
pCMV6-PFAS-T619D	This study	NA
pCMV6-PFAS-S215A-T619A	This study	NA
pLenti-III-PGK-PFAS	This study	NA
pLenti-III-PGK-PFAS-S215A-T619A	This study	NA
p3xFlag-CMV7-Erk2-L73P-S151D (ERK2-CA)	This study	NA
p3xFlag-CMV7-Erk2 KR (ERK2-KD)	Addgene	39224
Software and Algorithms
ImageJ	Schneider et al., 2012	RRID: SCR_003070
SCANSITE 4.0	Obenauer et al., 2003	https://scansite4.mit.edu/4.0/#cite
Prism 7	Prism	https://www.graphpad.com/scientific-software/prism/
Other
Multipurpose Scintillation Counter	Beckman	Model: LS 6500
Microplate Reader	Tecan	Infinite M1000 Pro
Nitrocellulose membrane 0.2 μm	GE Healthcare	10600001