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. 2020 May 16;104(13):6023–6043. doi: 10.1007/s00253-020-10668-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — PCA clustering of the aerobic and oxygen-limited naphthalene-amended biofilm enrichments on the basis of 16S rRNA gene–based T-RFLP electropherograms. (BF, initial biofilm (black spot); NAF_A, naphthalene-amended aerobic enrichments (blue spot); NAF_H, naphthalene-amended oxygen-limited enrichments (red spot); A/B replicates; numbers 1–6, number of weeks of enrichment; *, enrichment cultures selected for Illumina 16S rRNA amplicon sequencing together with the initial sample BF. Please note that in the case of aerobic enrichments of the first and third week, we were able to isolate the community DNA only from one replicate (NAF_A_A.1 and NAF_A_A.3, respectively)