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. 2020 May 1;104(13):5801–5812. doi: 10.1007/s00253-020-10627-7

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© The Author(s) 2020

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Fig. 2 — Temperature tolerance with temperature optimum of RpOhy. Reactions (triplicates) were carried out in 20 mM acetate buffer (pH 5) for 5 h at the respective temperatures. Reaction conditions: 5 μM purified RpOhy, 500 μM oleic acid with 1% DMSO as co-solvent and 20 μM FAD. Relative activity is based on the highest absolute activity (here at 25 °C) and set to be 100%