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. 2020 May 1;104(13):5801–5812. doi: 10.1007/s00253-020-10627-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — pH tolerance with pH optimum of RpOhy. Reactions (triplicates) were carried out at 25 °C for 5 h at the respective pH values. Reaction conditions: 5 μM purified RpOhy, 500 μM oleic acid with 1% DMSO as co-solvent and 20 μM FAD. Relative activity is based on the highest absolute activity (here in 20 mM acetate buffer, pH 5) and set to be 100%