Table 1.
Tested substrates and position of hydroxyl group in product of RpOhy (DSM 20415) and ReOhy (PR4) whole-cell substrate screening. All reactions were performed in triplicate
| Entry | Substrate | RpOhya | ReOhy (PR4)a | ReOhy (CCM2595)b | |
|---|---|---|---|---|---|
| 1 | Myristoleic acid | 14:1, cis-9 | 10 | 10 | --c |
| 2 | Palmitoleic acid | 16:1, cis-9 | 10 | 10 | 10 |
| 3 | Oleic acid | 18:1, cis-9 | 10 | 10 | 10 |
| 4 | Linoleic acid | 18:2, cis-9,12 | 10 | 10 | 10 |
| 5 | Pinolenic acid | 18:3, cis-5,9,12 | 10 | 10 | Unknownd |
| 6 | cis-Vaccenic acid | 18:1, cis-11 | --c | --c | --c |
| 7 | trans-Vaccenic acid | 18:1, trans-11 | --c | --c | unknownd |
| 8 | γ-Linolenic acid | 18:3, cis-6,9,12 | 10 | 10 | 10 |
| 9 | Ricinoleic acid | 18:1, cis-9, (R)-12-OH | 10 | --c | unknownd |
| 10 | cis-11-Eicosenoic acid | 20:1, cis-11 | 12 | 12 | unknownd |
| 11 | cis-8,11-Eicosadienoic acid | 20:2, cis-8,11 | --c | 12 | unknownd |
| 12 | cis-8,11,14-Eicosatrienoic acid | 20:3, cis-8,11,14 | --c | 12 | --c |
| 13 | cis-5,8,11,14-Eicosatetraenoic acid (arachidonic acid) | 20:4, cis-5,8,11,14 | --c | 12 | --c |
| 14 | Erucic acid | 22:1, cis-13 | --c | 14 | unknownd |
| 15 | Nervonic acid | 24:1, cis-15 | --c | --c | unknownd |
aPosition of hydroxyl group determined by GC-MS.
bPosition of hydroxyl group and substrate acceptance determined by Lorenzen et al. (2017)
cNo water addition
dNot tested