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. 2020 Jun 12;2020:9809632. doi: 10.1155/2020/9809632

Figure 3.

Figure 3

The enhanced cellular proliferation and invasion by circ-SETD2 are related to miR-519a/PTEN. miR-519a provides binding sites with circ-SETD2 and PTEN (a). The mRNA levels of miR-519a and PTEN were quantified by qRT-PCR, and Western blotting was used for analysis of protein expression of PTEN (b). The binding between miR-519a and PTEN was confirmed by luciferase assay (c). The mRNA levels and protein levels of PTEN in miR-519a-overexpressed or miR-519a-downregulated HTR8/SVneo cells were measured by qRT-PCR and Western blotting, respectively (d). The proliferation and invasive activity of HTR8/SVneo cells were respectively examined by CCK-8 kit (e) and transwell assay (f) after transfection of cells with miR-519a mimics or inhibitors. Relative genes expression was normalized by GAPDH expression. The data are represented as the mean ± S.D. (n = 3). ∗p < 0.05; ∗∗p < 0.01.