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. 2020 Apr 30;69(7):1389–1400. doi: 10.2337/db19-1218

Figure 6.

Figure 6

Juvenile skeletal muscle in vivo insulin response. Insulin-responsive protein phosphorylation (p) measured by Simple Western in gastrocnemius biopsies collected before (basal) and 10 min after (stimulated) intravenous insulin injection; IRS1 pY896 (A), Akt pS473 (B), GSK3β pS9 (C), total GSK3β (D), and AS160 pT642 (E). Abundance expressed as a ratio of protein peak area relative to Actin or GAPDH. F: Example Simple Western probing of protein abundance. Data were analyzed by two-way ANOVA with Tukey multiple comparisons test. Brackets indicate group comparisons (*P ≤ 0.05, n = 4 in CON and 5 in WSD).