Fig. 5.
a–d: Longitudinal hippocampal volume measurement. a. Representative coronal T2-w MR image of control mouse hippocampus (red insert) following ROI analysis in ImageJ. b. Sagittal T2-w MR image where red line indicates approximately where the coronal slice was acquired through the mouse brain. c. Representative T2-w images displaying the hippocampus from the same control and mdx mice between 6 and 18 months old. No gross anatomical differences were observed. d. Bar graph displaying relative hippocampal volume at all-time points investigated. Relative hippocampal volume is calculated based on the total volume of the hippocampus expressed as a percentage of total brain volume. We detected no changes in the relative hippocampal volume between control and mdx mice between 6 and 18 months old (p > 0.05). Data presented as mean ± SEM following students t-test for control vs. mdx mice, n = 8 mice per genotype at each time point.