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Development of SpyCatcher immune receptor targeting ligands. (A) Schematic representation of Protein G-ST cross-linking to clinical-grade human IgGs. (B) Cross-linking of Herceptin with Protein G-ST or Protein G-STDA, followed by subsequent reaction with SpyCatcher-Venus analyzed by SDS-Page gel under reducing conditions with coomassie staining. (C) DARPin9.26-ST and DARPin9.26-STDA reacted with SpyCatcher-Venus analyzed by SDS-Page gel with Coomassie staining.
