Figure 3.
MALDI-MS spectra of N-glycans (Na+ adduct) released from BTG: mdSUGAR labeled with periodate oxidation (A). High mannose (green label) and fucosylated (red label) N-glycans showed almost identical spectral profile prior to (B) and after (C) periodate oxidation due to lack of sialic acid. Highlighted acidic N-glycans (purple labels) showed mass loss before (D) and after (E) periodate oxidation. Mass shift of 62 Da was illustrated for each sialic acid after periodate oxidation. Star represents N-glycans as [M-H+2Na]+.