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Relative quantification performance of triplex mdSUGAR-labeled N-glycans released from BTG. Labeled N-glycans were mixed at ratios of 1:5:10 and analyzed in triplicate. The peak areas of M/L and H/L are plotted. Box plots show the median (line), the 25th and 75th percentile (box), and the 5th and 95th percentile (whiskers) (A). Representative extracted ion chromatograms for mdSUGAR-labeled N-glycans H5N2 (B) and H5N4S (C) are shown.
