Table 2.

Results of the last 50 ns molecular dynamic simulation for the fusion proteins. Data represent mean ± SD.

Parameters	p28-Linker-IL-24 fusion protein	p28-Linker-M4 fusion protein
Protein backbone RMSD	0.98 ± 0.01	1.36 ± 0.08
Protein backbone RMSF	0.43 ± 0.29	0.418 ± 0.21
Rg	1.67 ±0.006	2.001 ± 0.082
Temperature (Kº)	300.07 ± 1.51	299.9 ± 1.66
Potential (KJ/mol)	-488416 ± 732.03	-398516 ± 659.01
Total energy (KJ/mol)	-397398.264 ± 900.94	-324549 ± 813.10
Kinetic (KJ/mol)	91017.55 ± 460.83	73969.55 ± 409.59
H-bond pro-sol	369.11 ± 11.56	299.30 ± 11.09
Solvent accessible surface	99.69 ± 1.76	91.62 ± 2.0291
Minimum distance between of two peptides	0.17 ± 0.01	0.20 ± 0.020

RMSD, Root mean square deviation; RMSF, root mean square fluctuation; Rg, radius of gyration.