Fig. 6.

Western blot analysis of the supernatants from CHO-s cells transfected with recombinant plasmid harboring either wild-type or codon-optimized hIFN-β sequence. Lane 1, protein molecular weight marker; lane 2, hIFN-β expressed by the wild-type sequence; lane 3, hIFNβ expressed by codon-optimized sequence; and lane 4, untransfected cell culture medium as negative control. The two arrows, showing the glycosylated and non-glycosylated forms of hIFN-β, indicating the heterogeneity of the hIFN-β glycoforms. CHO-s, suspension adapted Chinese hamster ovary; hIFN-β, the recombinant human interferon beta.