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. 2020 Jun 21;85:104421. doi: 10.1016/j.meegid.2020.104421

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© 2020 Elsevier B.V.

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Fig. 4 — Genetic characterization of Anelloviridae family members detected in the present study. (A) Genomic organization of LV05 detected in Cerdocyon thous in the present study. (B) Genomic organization of LV07 detected in Lycalopex gymnocercus in the present study. (C) Amino acid phylogenetic tree of complete ORF1 gene highlighting the sequences obtained in the present study. The sequences were analyzed through the maximum-likelihood method with JTT + G + I model. All analyses were conducted with 1000 bootstrap replicates, and the percentage of replicate trees in which the sequences clustered together have been presented adjacent to the branches. Bootstrap values for each node have been shown if they were > 50%. The sequences detected in the present study has been highlighted with ● for sequences detected in Cerdocyon thous, and with ▲ for sequences reported in Lycalopex gymnocercus. The sequences detected in the present study were deposited in GenBank database: CtTTV-1 LV05 (MT010524), CtTTV-2 LV15 (MT010525), CtTTV-3 LV08 (MT010526), CtTTV-4 LV23 (MT010527), LgTTV-1 LV06 (MT010528), LgTTV-1 LV07 (MT010529), and LgTTV-2 LV13 (MT010530).

Fig. 4 — Genetic characterization of Anelloviridae family members detected in the present study. (A) Genomic organization of LV05 detected in Cerdocyon thous in the present study. (B) Genomic organization of LV07 detected in Lycalopex gymnocercus in the present study. (C) Amino acid phylogenetic tree of complete ORF1 gene highlighting the sequences obtained in the present study. The sequences were analyzed through the maximum-likelihood method with JTT + G + I model. All analyses were conducted with 1000 bootstrap replicates, and the percentage of replicate trees in which the sequences clustered together have been presented adjacent to the branches. Bootstrap values for each node have been shown if they were > 50%. The sequences detected in the present study has been highlighted with ● for sequences detected in Cerdocyon thous, and with ▲ for sequences reported in Lycalopex gymnocercus. The sequences detected in the present study were deposited in GenBank database: CtTTV-1 LV05 (MT010524), CtTTV-2 LV15 (MT010525), CtTTV-3 LV08 (MT010526), CtTTV-4 LV23 (MT010527), LgTTV-1 LV06 (MT010528), LgTTV-1 LV07 (MT010529), and LgTTV-2 LV13 (MT010530).