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. Author manuscript; available in PMC: 2021 Mar 19.
Published in final edited form as: Reprod Toxicol. 2020 Mar 19;92:4–10. doi: 10.1016/j.reprotox.2020.03.006

Figure 2. Omics Scale Data for Exposome Epidemiology.

Figure 2.

Criteria for omics scale data are provided on the left based upon success of the Human Genome Project in providing genomics data meeting these criteria. For high-resolution metabolomics (HRM), a single step sample treatment was found to effectively remove protein and prepare sample for analysis by liquid chromatography with ultra-high-resolution mass spectrometry detection. By use of an autosampler, a column switching valve and a short run time, a relatively high throughput analytic capability was obtained. The assay gives extensive coverage, with routine detection of >30,000 mass spectral features. Analyses with three technical replicates enables reproducible data detection for each feature. With concurrent analysis of a pooled reference standard, a single point calibration method, reference standardization, provides quantitative analysis which are suitable for inclusion into cumulative data libraries. The current cost is approximately $125/sample.