Table 3.
MIDAS enrichment factor (EF) and binding affinity for RNAs
| RNA | EFa | Kd (nM) | |
|---|---|---|---|
| Nova-2 KH3 | |||
| UUUCA | 17.3 | 179 ± 23 | |
| UUCAC | 14.7 | 739 ± 82 | |
| UUUAA | 17.5 | 1038 ±120 | |
| UCACC | 2.8 | 2991 ± 450 | |
| CCACC | 1.1 | 6991 ± 1848 | |
| FMRP KH0 | |||
| UCACC | 0.9 | > 10,000b | |
| CCACC | 0.6 | > 10,000b | |
| GACUC | 0.2 | > 10,000b | |
| UUCAC | 0.2 | > 10,000b | |
| UUUCA | 0.1 | > 10,000b | |
| FMRP KH1-KH2 | |||
| UCACC | 0.5 | > 10,000b | |
| CCACC | 0.3 | > 10,000b | |
| GACUC | 0.3 | > 10,000b | |
| UUCAC | 0.3 | > 10,000b | |
| UUUCA | 0.1 | > 10,000b |
a
Enrichment factors were normalized to the relative abundance of selected RNAs from the EMSA gel
b
Estimated affinity based on EMSA selection using 10 μM FMRP KH domains